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The Petrache Lab at National Jewish Health is currently seeking a Lab Researcher to join our team. Our laboratory investigates pulmonary diseases such as COPD, and mechanisms of lung injury and repair. The research projects focus on the role of sphingolipid metabolism, cigarette smoke and e-cigarette vapor, and susceptibility to viral or bacterial infections.

The methods used in our laboratory include tissue culture, exposure of cells to cigarette smoke and e-cigarettes, isolation of cells from murine and human tissues, transfections, RNA and protein isolation, quantitative PCR, Western blotting, histology, immunofluorescence, fluorescence microscopy, flow cytometry, and mouse models of lung disease. Training in these methods will be provided.

Candidates who have been exposed to basic research methods through coursework, internships, or another job and show enthusiasm for basic science research are preferred.

The selected candidate for this position will:

Work under the supervision of principal investigator and senior lab personnel. Be involved in daily collaborative interaction with other lab members. Perform experiments independently after receiving training. Collect, analyze and graph the data using basic statistical software (Microsoft Office Suite, GraphPad Prism). Present data on lab meetings, with option of presenting at institutional as well as regional and national scientific meetings. Be highly organized and able to maintain clear and reliable record, including computer databases. Participate in laboratory maintenance & operations.

Position Summary The position is responsible for planning, developing, executing and monitoring testing techniques and investigative procedures under the direction of a Principal Investigator or Lab Manager.

Essential Duties

Develops new techniques and investigative procedures, including scientific routines, computer programming techniques and data reporting techniques. Monitors quality control of routines, experiments, general laboratory activities and the use of research animals in the laboratory setting. Performs laboratory functions unique to the assigned laboratory. Provides general guidance to laboratory staff, including technicians, support and auxiliary staff. Acts as a leader for laboratory programs and staff in assigned areas. Demonstrates enhanced proficiency in data analysis, preparation of glassware, extraction and plating procedures, programming and mathematical ordering systems. Prepares statistical tabulations, using a calculator or computer. Oversees supply functions, inventory control, equipment use and maintenance, and animal care and ordering, as needed. Maintains compliance with all institutional / safety training requirements. Maintains laboratory records, supplies, samples in an organized manner (such as a laboratory notebook and sample storage record). Acts a liaison to other department personnel, outside investigators, and vendors. Performs routine and non-routine tests leading to the successful completion of developed experiments. Other Duties

Performs all other duties as assigned. Competencies

Accountability: Accepts full responsibility for self and contribution as a team member; displays honesty and truthfulness; confronts problems quickly; displays a strong commitment to organizational success and inspires others to commit to goals; demonstrates a commitment to National Jewish Health. Laboratory Knowledge: Demonstrates and understands laboratory testing and methodology. Decision Quality: Makes good decisions based upon a mixture of analysis, wisdom, experience, and judgement. Sought out by others for advice and solutions. Adaptability: Maintains effectiveness in the midst of change in work responsibilities or environment, requirements ambiguity, or stress. Problem Solving: Uses issue identification, data relation and comparison, pattern recognition and information gathering to create analyses and make recommendations. Informing/Communicating: Conveys ideas, responses, or instructions effectively, appropriately, and persuasively through writing or speech. Professional Relationships: Interacts with others in a constructive, positive, and respectful manner, regardless of individual differences. Assists team members or co-workers in achieving personal goals and completing assignments. Drive for Excellence: Consistently meets performance expectations, ensures that accurate, objective, and timely measures of quality are made and used. Innovation Management: Identifies key trends, opportunities, and organizational capabilities that would bring competitive advantage to the organization. Sees trends before others and integrates concepts in a unique wa Supervisory or Managerial Responsibility None

Travel None

Core Values

Be available to work as scheduled and report to work on time. Be willing to accept supervision and work well with others.